The working principle of probe of DO/O2 Sensor is polarographic measurement. The probe is made of anode, cathode and membrane. During using, immerse the probe into solution to be measured. Electrical potential is applied on the anode and cathode so that electrochemical reaction is occurred. DO/O2 Sensor also can be used to measure concentration of oxygen in air.
Specification
| Range |
0~20mg/L |
|
Accuracy |
±0.5mg/L |
|
Resolution |
0.01mg/L |
Usage
Connect DO/O2 Sensor to any port in SenseDisc.
Screw off the cover of DO/O2 Sensor probe. Rinse the probe cover and metal tube using deionized water. Swing to dry them respectively.
Inject filling liquid into cover to fill three quarters of volume of it. Screw back the cover tightly.
Wipe out the waterlogging on the probe. Touch the membrane at the top of cover to check if it is touching completely to the metal tube. There should be no gas bubble or solution interlayer, otherwise you have to install the cover again.
Make sure the indicator of sensor is light up. SenseDisc is conducted and turned on. Wait for 45 minutes.
Calibration: configure anaerobic water. Immerse probe into anaerobic water. Wait until the instrument display becoming stable and calibrate the probe.
Full calibration: if the accuracy required in experiment is not high, you can calibrate it in air. After polarization, you can start full calibrating. If the accuracy required in experiment is high, you should calibrate it in saturated water. After polarization, immerse the probe into dissolved oxygen deionized water with constant temperature. Wait until the instrument display becoming stable. The full calibration is completed.
Place the sensor probe into the environment to be measured to collect data.
Typical experiment
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Explore breathing regulation of saccharomycetes
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Explore DO concentration in water with different quality
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Factors affect photosynthesis of plant
Note
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Do not touch it to grease or other organic matter.
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If there is incrustation on the probe, immerse it in 10% diluted hydrochloric acid for 3 minutes. Rinse it using water. If it is polluted by organic matter, immerse it in 80% ethyl alcohol for 10 minutes and rinse it using water.
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When using polarographic DO probe to measure liquid (include using saturated dissolved oxygen deionized water to full calibrate it), it is required that the liquid has mobility. There are two methods to make the liquid available:
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When measure static or slow flowing liquid, pinch the probe and waggle it horizontally with speed of 20~30 cm/s.
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You can use constant mixer. Adjust the mix speed to ensure the instrument display stable. After that, you can start full calibrating or measuring.
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The gas-permeable membrane can not be damaged. If it is broken of after long time using, please replace it with a new one.
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The filling liquid in the cover must be replaced periodically. The period depends on the frequency and condition of using. When the quality is low or the probe can not work, please change the filling liquid first. If the problem is not solved, please operate according to maintenance.
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